Cat No. N9066, 1g
Specific Activity: ≥ 2000 U/mg
Form: Contains 1g DNase I, Lyophilized Powder
Store at -20°C for 2 years.
DNase I is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA: DNA hybrids.
• Extracted from the bovine pancreas.
• Degradation of DNA template in transcription reactions
• Removal of contaminating genomic DNA from RNA samples
• DNase I footprinting
• Nick translation
Definition of Activity Unit
One unit of the enzyme completely degrades 1 µg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 µg of pUC19 DNA.
38 kDa monomer.
Functionally tested for digestion of template DNA after in vitro transcription.
Inhibition and Inactivation
Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM.
Inactivated by heating at 65°C for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+).
DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.